Примеры использования Fluorescent protein на Английском языке и их переводы на Русский язык
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Fluorescent proteins of the red spectral region.
His lab largely focuses on fluorescent proteins and biosensors.
A solution of BDP TR methyl ester,a counterstain for cells expressing green fluorescent protein GFP.
For this purpose fluorescent proteins are widely used as markers of caspase-3 activation.
YFP based indicators are mutated forms of Green fluorescent protein GFP.
Fluorescent proteins and biosensors based on them will be the key investigative tools in this project.
The neurons of such animals synthesized fluorescent protein red and accessory cells of nervous tissue(called microglia)- green.
Biologists are working on a model object- zebrafish brain-rerio(zebrafish, Danio rerio),the genome of which were made of fluorescent protein genes.
In particular, there has been developed a sensor consisting of fluorescent proteins YFP and CFP to determine Bid activation and apoptosis induction 103.
Red and far-red fluorescent proteins have offered fundamentally new prospectsfor monitoring normal and pathological processes in the whole organism.
To assess this the researchers infected cell culture using LeGO vectors,each of which contained a fluorescent protein with one of the three basic colors.
Newly developed fluorescent proteins that absorb in the near-IR range(e.g. red fluorescent protein) allow imaging deep inside tissues.
In a visual assay utilizing transgenic marker constructs it is possible to encode pollen-expressed fluorescent proteins of three colors in the quartet mutant background.
Once fluorescent proteins had been discovered, it became possible to study gene expression, localization and functional activity of proteins in living cells 4, 5.
After optical excitation, the fluorescence of organic dyes, fluorescent proteins or quantum dots are detectable in the low-loss window for biological tissue 1000- 1700 nm.
Comparison of sensibility of the two cell lines to the effect of cisplatin showed that transfection of the cells with the fluorescent protein results in the increase of the sensitivity to cisplatin.
Yellow fluorescent protein(YFP) is a genetic mutant of green fluorescent protein(GFP) originally derived from the jellyfish Aequorea victoria.
Recently, there has been suggested a sensor to study simultaneously the activity of caspase-8 andcaspase-3 consisting of fluorescent proteins seCFP, Venus and mRFP1 bound by the linkers IETD and DEVD 70.
It has been previously shown that when fluorescent protein tracers are injected into the cervical ganglion in order to bypass the circulatory system, they are not found on the neuron surface.
These techniques initially relied upon the direct physical injection of various visualizable tracer molecules(e.g. Green fluorescent protein, lipophylic dyes or radioactively tagged amino acids) into the brain.
Encoding sequence of the fluorescent protein mCherry was obtained by treating pIG6-4D5scFv-mCherry with AscI restriction endonuclease[18] and ligated with plasmid pDARP-PE40[17], pretreated with the same endonuclease.
Corals and jellyfish are the source of a series of proteins, generally known as Green Fluorescent Proteins, which were developed as a"reporter" system for protein expression in animals.
A useful technique for assessing cellular localization uses genetic engineering to express in a cell a fusion protein orchimera consisting of the natural protein of interest linked to a"reporter" such as green fluorescent protein GFP.
Keywords: mesenchymal stem cells; adipose-derived adult stem(ADAS) cells;red fluorescent protein Turbo FP635; tumor Hela Kyoto; vital bioimaging; laser scanning microscopy; transfection; carcinogenesis.
After nanocutting of nerveendings innervating moving back, functional resroration of the nerve fibers cut was shown using fluorescent proteins for neuron labelling.
An increased sensitivity of the cells transfected by GFP or its analogs(yellow fluorescent protein or enhanced GPF) to antitumor preparations is closely linked with the production of ROS, singlet oxygen in particular 11.
Animal tissues are known to be more transparent in red and near infrared spectral range, therefore,in recent years, researchers have aimed their efforts at developing fluorescent proteins and FRET-sensors working in this range.
This transgenic approach is not limited to fluorescent proteins: infecting tissue with a vaccinia virus carrying the tyrosinase gene allows in situ production of melanin, which generates strong optoacoustic signal for MSOT.
In 1999, Dr. Zhiyuan Gong and his colleagues at the National University of Singapore were working with a gene that encodes the green fluorescent protein(GFP), originally extracted from a jellyfish, that naturally produced bright green fluorescence.