Exemplos de uso de Amplified by pcr em Inglês e suas traduções para o Português
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Figure 3: A simplified diagramshowing how defined DNA fragments are amplified by PCR.
The fragment of the hla-g gene was amplified by pcr, further purified and sequenced.
Genotyping was performed by pcr-rflp,with exception for the vntr of gene dgat1that was only amplified by pcr.
Thelectin gene bfl was amplified by PCR from genomic DNA, and cloned to expressionvectors pQE30 e pET32a.
Exons 1 to 3 of prop1 gene, comprising the entire coding region,were amplified by pcr using intronic primers and sequen.
The bfl lectin gene was amplified by pcr from genomic dna make and subsequently cloned into the expression vectors pet32a.
The dna was isolated using the phenol-chloroform protocol, the mitochondrial andnuclear region regions were amplified by pcr.
Each bead has its own DNA fragment template,and these are amplified by PCR, and then placed in wells on a glass slide.
The dna was isolated using a standard phenol-chloroform protocol and mitochondrial regions andmicrosatellite loci were amplified by pcr.
The entire bfpa gene and a portion of the intimin gene were amplified by pcr from epec genomic dna and inserted into the pmip12 vector at the bamhi/kpni sites.
This region as well as a smaller region containing half of the putative promoter(546 bp)were amplified by PCR using specific primers.
Phtd and phtd_cter genes were amplified by pcr from genomic dna of streptococcus pneumoniae d39 strain, cloned into pcr¿2.1 vector and subcloned in pqe-30.
The exons corresponding to coding regions of the three genes investigated were amplified by PCR using the primers previously described.
The jun protein cdna was amplified by pcr and cloned into pet 26b(+), pet 28a-c(+) and p1813 expression vectors, and was expressed in e. coli bl21 de3.
In order to verify the presence of ADSCs within the kidneys after treatment,the expression of SRY mRNA was amplified by PCR in real-time Figure 1U.
The coding sequences of hg-csf and higf-1 were amplified by pcr and subcloned into a lentiviral transfer vector containing a constitutive promoter.
Viral DNA was extracted by DNAzolTM, TRIzolTM and/or QIAampTM DNA mini Kit methodologies andb-globina gene was amplified by PCR as a control reaction.
CDNA samples obtained by reverse transcription were amplified by PCR with primers under the same conditions as described by Sandres-Sauné et al22 2003.
Total blood samples of 32 patients(21 with phenylketonuria; 11 with non-pku hpa) were submitted to dna extraction and, afterwards, the whole gene pah codifying region(13 exons andadjacent regions) was amplified by pcr and analyzed through dna direct sequencing.
The fragments amplified by PCR were respectively submitted to RFLP analysis, using 5 U of the following enzymes: DdeI enzyme, BGlII, BfaI, FauI and MspI New England Biolabs.
The gene encoding the s. maltophilia cgtase,was successfully amplified by pcr, cloned into the vector pet-23a(+) and expressed in escherichia coli bl21de3.
After the forest inventory was evaluated the floristic composition, the phytosociological parameters and spatial distribution of bamboo and for genetic diversity studies, plant material was collected, used for dna extraction,which was amplified by pcr using 8 issr markers.
Initially, the extraction of dna of 84 samples formalin-fixed paraffinembedded was performed. the samples were amplified by pcr. sequencing was utilized to analyze the v600e. the v600e mutation was not de.
The bound sequences are eluted and amplified by PCR to prepare for subsequent rounds of selection in which the stringency of the elution conditions can be increased to identify the tightest-binding sequences.
Str markers are currently the most commonly used genetic markers in forensic genetics due to their high polymorphism, high reproducibility,possibility of being amplified by pcr in multiple copies in a single reaction, and minute quantities of dna 1ng.
The dna sequence of epitopes encoded by hivbr18 vaccine was amplified by pcr and cloned into a plasmid that contained the sequence of gd, giving rise to plasmid pvax-gdh-hivbr18.
The phylogenetic analysis of the NS5B region of the virus genoma was performed by confirming HCV genotypes andsubtypes. cDNA samples obtained by reverse transcription were amplified by PCR with primers under the same conditions as described by Sandres-Sauné et al 2003.
For this, dna from six species of the genus was extracted and amplified by pcr using sequences from the rbcl andmatk genes and the trnh-psba spacer gene, followed by sequencing using the sanger method.
The its and tef-1¿regions were amplified by pcr(polymerase chain reaction), sequenced and compared in two databases, which allowed the identification of 60 isolates to the species level most identified as t. harzianum.
Genomic dna soil, treatments, and the fragment was extracted from bacteria andarchaeal 16s rdna was amplified by pcr, the sequence being sequenced by pyrosequencing using the life technologies ion platform torrentetm.