Exemplos de uso de Amplified using em Inglês e suas traduções para o Português
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DNA can be amplified using a procedure called the polymerase chain reaction PCR.
For molecular analyzes dna from 60 apple accessions was extracted and amplified using issr primers.
Microsatellite regions were amplified using seven primers per species, previously developed.
The partial region of the genes comprising the issr andtransposon were amplified using seven primers.
The HPV-DNA was amplified using the MY9 and MY11 universal primers, which amplify the large majority of HPV types.
Another method is multiple displacement amplification,where the whole genome can be amplified using small levels of genomic DNA.
The gene sequence of nagt was amplified using primers(l0 and l5) designed and optimized for the external regions of the gene nagt generating a fragment of approximately(≈) 1706 bp.
The proximity orinteraction of two proteins can lead to ligation of DNA molecules which can then be amplified using a polymerase chain reaction.
In 1987, DNA from a strand of human hair was amplified using PCR and this confirmed the ability of PCR to amplify DNA present in degraded samples part of forensic evidence.
The samples were stored in a freezer at -20°C. Genotyping of the SNPs rs4644 and rs4652 of the galectin-3 gene was performed by real-time polymerase chain reaction PCR using TaqMan® Life Technologies, Carlsbad, USA, andthe SNPs were amplified using the TaqMan® Universal PCR protocol Life Technologies.
Total dna was extracted from each sample andcomplete viral genomes were amplified using the dna polymerase from phage phi29, cloned into plasmid vectors and completely sequenced.
They were conditioned and amplified using Miotool equipment Miotec with eight input channels, 14-bit resolution A/D converter in the acquisition of EMG signals, 5,000 V electrical insulation, maximum acquisition capacity of 2,000 samples/second/channel, and 20 Hz high-pass and 500 Hz low-pass filters.
Genomic DNA, isolated from peripheral blood according to the standard method,was amplified using the polymerase chain reaction PCR protocol, as previously described.
The exons of the SCN5A gene were amplified using conditions that we established: 0.2 mM dXTP, 3 mM MgCl2, 0.5 µM of primers sense+ antisense, 0.625 IU of recombinant Taq DNA polymerase Invitrogen, 200 ng of DNA template, in a final volume of 50 ml.
In this context, we aimed at analyzing the genomes of isolates collected in different producing regions of the country,based on its enzyme restriction profile and on the sequence of the rt/rnase h. the viral dsdna was obtained with the extraction of the total dna of the plants, from the foliar tissue and amplified using the ill.
The DNA of leucocytes extracted was amplified using specific primers for the identification of the mutation with posterior digestion with restriction enzyme Dde I. It was visualized in poliacrylamide gel at 12%, with intronic primers that flank the area of the exon 19 of the gene CFTR.
Exons of genes KCNQ1 and KCNH2 were amplified using, respectively, the programs"B" and"C" previously published by Syrris et al To amplify exons 1A of KCNQ1 and 4 of KCNH2, the PCRx Enhancer reagent Invitrogen was added to the reaction, according to manufacturer's instructions.
A 352-bp fragment of the 5-HT2A gene was amplified using primers HT2AFWD 5'-CCT CAT CTG CTACAAGTT CTG GCT T-3'and HT2A-REV 5'-GCA TTC TGC AGC TTT TTC TCT AGG G-3', modified from Gursoy et al. The primers described by Gursoy et al. were used to amplify a 185-bp fragment of the COMT gene.
The -262A> T ACE polymorphism was amplified using 0.3 pmol each primer forward, 5'-TCG GGC TGG GAA GAT CGA GC -3' and reverse, 5'-GAG AAA GGG CCT CCT CTC TCT-3' synthesized by Invitrogen-Life Technologies, São Paulo, SP, Brazil, in which the underlined nucleotide represents the deliberated primer mismatch designed to introduce an artificial restriction site.
An acoustic guitar can be amplified by using various types of pickups or microphones.
Guajava was collected in goiatuba(go), lagarto(se) and ceará mirim(rn)and were amplified separately using 12 microsatellite pairs primers and seven issr primers.
Briefly, the RNA was reverse transcribed and amplified by using specific primers corresponding to a conserved nucleotide sequence of the VP4 and VP7 genes, fragments of 876 bp and 904 bp, respectively.
An earlier study using amplified fragment length polymorphism(AFLP) fingerprinting showed a close association of wisent and American bison and probably with yak, but noted that the interbreeding of"Bovini" species made determining relationships problematic.
The IPCC are using these amplified results from the faulty climate models and the NOAA faulty study to scare people into accepting their control.
The Acoustic Singer Pro has a unique vocal harmony function,allowing you to create live harmonies using your amplified vocals and/or guitar.
Of course, this is very different from detecting amplified STRs using electrophoretic automatic sequencing(and does not even accurately represent the visualisation of VNTRs using Southern blotting, as the DNA is stained directly on the gel), but it nonetheless demonstrates the principles of the analytical process.