Exemplos de uso de Applied biosystems em Inglês e suas traduções para o Português
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The data was analyzed by the GeneMapper v4.1 software Applied Biosystems.
The High Capacity cDNA Reverse Transcription kit Applied Biosystems, CA, USA was used to synthesize complementary DNA cDNA from 1000 ng of total RNA.
The PCR reaction was done using the PCR Taq-Man PE Applied Biosystems kit.
For each reaction,1.25 U of Taq polymerase was added GeneAmp®, Applied Biosystems, Branchburg, USA in an appropriate buffer, following the instructions on the kit.
AB1 files are Data Files primarily associated with ABIF Applied Biosystems Inc.
For allelic discrimination,the made-to-order TaqMan Applied Biosystems probes for rs17563, rs207147, and rs762642 SNPs, using 50 ng of DNA per sample, were used. rs762642 is located in an intronic region 14:54423053 of chromosome 14.
BigDye Terminator method was used on an ABI 3100 Applied Biosystems Inc USA.
Μl of the purified amplification product was transferred to sequencing tubes Applied Biosystems containing 12 µl of deionized formamide Applied Biosystems and 0.5 µl of ROX 50-500 DNA Ladder Applied Biosystems as internal size standard, denatured at 90ºC for 5 min and cooled on ice.
AB1 files are Data Files primarily associated with ABIF Applied Biosystems Inc.
The extracted DNA specimens were submitted to quantitative real-time PCR Applied Biosystems 7500 Fast Real-Time PCR System by using commercially available assays according to the manufacturer's instructions: HSV1 Q-PCR Alert AmpliMIX, HSV2 Q-PCR Alert AmpliMIX, VZV Q-PCR Alert AmpliMIX, EBV Q-PCR Alert AmpliMIX, Q-CMV Real Time Complete, HHV-6 Q-PCR Alert AmpliMIX Nanogen Advanced Diagnostics S.r. L, the HPV High Risk Screen Real Time PCR Sacace Biotechnologies and HPV 6/11 Real-TM Real Time PCR kit Sacace Biotechnologies.
The mass spectra were obtained using an ABI 4700 TOF/TOF Applied Biosystems device.
Quantitative measurements were made with a commercial kit TaqMan qPCR; Applied Biosystems in a detection system StepOne Plus; Applied Biosystems.
Real-time quantitative PCR was performed using an ABI 7500 Real Time PCR System Applied Biosystems.
We used the ABI PRISM 310,Genetic Analyzer Applied Biosystems, Perkin Elmer, California.
PCR protocols were performed in accordance with the TaqMan Genotyping Master Mix manufacturer's instructions Applied Biosystems, CA, USA.
Sequences obtained were edited with the Sequence Navigator program version 1.0 Applied Biosystems, Inc., CA, EUA and aligned using the program Megalign Lacergene, DNA STAR, Inc., WI, USA.
Thermal cycling was performed in a real-time PCR system StepOnetm; Applied Biosystems.
Sequence Detector Systems Version 1.3.1 software Applied Biosystems was used for data analysis.
The product of such amplification was submitted to RFLP techniques, according to the protocol proposed by Bernard et al. andsequenced by the Big Dye Terminator Cycle Sequencing kit Applied Biosystems, Foster City, CA.
All genotyping was performed in LIM-25-FMUSP, using the TaqMan methodology Real Time TaqMan® SNP single nucleotide polymorphisms Genotyping Assay C-8746719-20, Applied Biosystems, Carlsbad, United States,using the equipment for Real Time Applied Biosystems, model StepOnePlus Applied Biosystems, Carlsbad, United States.
Sequencing was performed on ABI Prism 377 DNA Sequencer device according to the Protocol described in the kits used Templiphi DNA amplification, Amersham Biosciences; Big Dye Terminator Cycle Sequencing Ready Reaction,PE Applied Biosystems.
The entire process was done by an ABIPRISM 7300 PE Applied Biosystems sequence detector.
The positive products were purified using the mega quick-spin total¿kit(intron biotechnology) and sequenced using the bigdye¿terminator v3.1 cycle sequencing kit(life technologies) andthe platform 3130xl genetic analyzer applied biosystems.
The reactions were performed using a DNA analyzer system Applied Biosystems, Foster City, CA, USA.
We selected one SNP, which was genotyped using TaqManr SNP Genotyping Assays Applied Biosystems, Foster City, CA.
Amplification was performed in an ABI PRISM 7000 SDS thermocycler Applied Biosystems, Foster City, CA, USA.
Cycle sequencing was carried out using the BigDyeTM kit, version 3.1 Applied Biosystems Inc., USA with primers Anti-A1 and RVG9.
These PCR plates are compatible with most thermal cyclers,including the Applied Biosystems 9600 and 9700, and the MJ Research PTC-100.
Gene expression was quantified in relation to the values of the C group after normalisation by an internal control cyclophilin:assay Rn 00690933_m1; Applied Biosystems by the method 22DDCT, as described previously.
The genomic fragments of HCV amplified through PCR technique were sequenced in ABI377 equipment(PE Biosystems®)using the"DNA sequencing kit Big Dye™ Terminator" from Applied Biosystems®, in accordance with the manufacturer's instructions.