Examples of using Two-photon in English and their translations into German
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Two-photon microscopy applications exploit the advantages of the technology.
The theoretical foundation of two-photon microscopy is two-photon excitation.
Two-photon microscopy provides deeper access to tissues than the confocal technique.
Scattered light affects the image qualities to a lesser extend in two-photon microscopy;
Two-photon microscopy produces fluorescence in the area where the laser beam is tightly focused.
One example is the procedure for performing two-photon in vivo imaging on the dorsal surface of mice brains.
Two-photon microscopy is a method that enables the imaging of live cell and tissue samples with high resolution.
As only specific planes are illuminated in two-photon microscopy, this reduces both photodamage and photobleaching.
The structure of the fluorescence detection path differs between conventional fluorescence microscopes and two-photon microscopes.
The point spread function in a two-photon microscopy is 0.3 Âμm in the radial direction and 0.9 Âμm in the axial direction.
Two-photon microscopy(also known as two-photon fluorescence light microscopy) was invented in 1990 as a new method of imaging live cells and tissues in three-dimensions.
Finally, there is no out of focus light generated when using two-photon microscopy, meaning there is less photobleaching and photo-damage.
The structure of two-photon microscopes consists of combining the fundamentals of two-photon absorption with the application of a laser scanner.
In the region above and below the focal point, the number of photonsare not high enough to ensure two-photon excitation, thus there is no fluorescence in regions other than the region of focus.
The basic principles of two-photon absorption have been applied to form a technique with unique advantages for imaging in vivo samples.
For the experimental implementation of ESR-channel,It was necessary to the coexistence of three sputannogo and two-photon scattering, that was carried out by two groups of researchers-Vienna, led by Anton Cojlingerom, and Roman, under the direction of Francesco de Martini.
Two-photon microscopy differs from conventional fluorescence microscopy as the excitation wavelengths of the two photons are longer than the resulting emitted light.
A mode of STED microscopy, known as two-photon excitation STED, enables imaging in thicker tissue samples such as brain slices.
Two-photon microscopy differs from conventional fluorescence microscopy by necessitating two photons to be absorbed by the fluorophore simultaneously, as well as through the excitation wavelengths of the two photons that are longer than the resulting emitted light.
One of the main advantages to two-photon microscopy is that the long excitation wavelengths from the two photons are less damaging to the specimen.
Therefore, we use in vivo two-photon imaging over time periods of months to analyze structural and functional changes during disease progression.
The infrared excitation light used in two-photon microscopy is less harmful to biological samples with UV-excitable fluorophores when compared to methods utilizing UV light.