Examples of using Reference sera in English and their translations into Spanish
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Official
Other candidate reference sera for sheep are available at Panaftosa.
Critical to accomplishing this task is the acquisition of standard reference sera.
Freeze-dried positive and negative reference sera is available as internal controls. Categories.
For serological validation, there is a need for positive andnegative camelid reference sera;
Information on potential candidate reference sera for other selected diseases is expected by April 2017.
Dr Renström had reported on the project to develop new reference sera for EBL.
It was determined that calibration reference sera are useful as primary international reference sera.
Reference sera and control materials as described in the Terrestrial Manual are also available from the Reference Laboratory.
The Commission received further clarification of the application of the existing OIE Reference Sera for different classes of serological assay.
Selection of European Union standard reference sera for use in the serological diagnosis of infectious bovine rhinotracheitis. 13(3), 947.
As IFA test performance mayvary in different laboratories, it is better for each laboratory to define its own threshold titre using defined positive and negative reference sera.
Reference sera to standardise FMD SP serological tests for some serotypes and subtypes are available from the Reference Laboratory at Pirbright.
This would meet the Commission's requirement for the inclusion of standard reference sera in the list of OIE-approved International standard sera. .
Using this index method, standard reference sera for all three host species of interest have been prepared, as well as evaluation panels for these species.
For end-point titration tests, duplicate twofold dilution series of positive andnegative homologous bovine reference sera should be included on at least one plate of every run.
Where reference sera are intended for different tests and purposes, it may be appropriate to maintain and distribute a single strong positive and a single negative reference serum.
In the future, it is hoped that new test kits coming onthe market will have made use, during their development and standardisation, of the reference sera and evaluation panels that have been prepared.
The serum samples included the three European reference sera EU1(antibody positive), EU2(antibody weak positive and defined as borderline sample) and EU3(antibody negative) Perrin et al., 1994.
The Commission welcomed this voluntary commitment from the OIE Reference Laboratory for these diseases in Italy,as standard reference sera for these diseases are not currently available in the list.
Additional reference sera are available in limited quantities from the OIE Reference Laboratories e.g. R1, R2 and R3 as positive, weak positive and very weak positive standard sera from the OIE Reference Laboratory in Germany.
The Commission would request the newly designated Collaborating Centre for Animal Protozoan Diseases in Japan to take forwardthe characterisation of an acceptable standard strain of Trypanosoma equiperdum and to develop internationally validated standard reference sera for dourine.
Guidelines on inactivation of adventitious agents Following reports from a number of Reference Laboratories,the Commission recognised that the current recommendation to use gamma irradiation for reference sera was not suitable for all applications due to apparent denaturation of the antibody activity.
Dr Selleck informed the Commission that work on the preparation of OIE international reference serum for the AI AGID1 test is well advanced.
Alternately, AGID antigen and reference serum may be prepared as described below.
The reference serum should be maintained as frozen aliquots in amounts sufficient for 1 week of tests.
Standardisation programme The Commission reviewed a technical report for an OIE inter-laboratory trial to determine the titre of the new batch of anti-rabies positive reference serum of dog origin.
The blocking ELISA titres of this reference serum against antigens prepared from the homologous vaccine strain and a field isolate are compared to determine how antigenically'similar' the field virus is to the vaccine virus.
As a last step, the porcine serum will be sent out along with the small ruminant serum(OIE reference serum for Brucella melitensis) by VLA Weybridge to the Reference Laboratories for testing by their in-house tests.
The blocking ELISA titres of this reference serum against antigens prepared from the homologous vaccine strain are compared with the corresponding titres of the serum against a field isolate to determine how antigenically‘similar' the field virus is to the vaccine virus.
The blocking ELISA titres of this reference serum against antigens prepared from the homologous vaccine strain are compared with the corresponding titres of the serum against a field isolate to determine how antigenically‘similar' the field virus is to the vaccine virus.