Homology Search By simply loading the genomic base sequence into the reference genome map,a database for Blast search is automatically created and homology search(Blast) search for that database can be easily performed.
Search Sequence and Annotation By simply loading the genomic base sequence into the reference genome map,a database for Blast search is automatically created and homology search(Blast) search for that database can be easily performed.
これで、Blast検索用データベースの作成が完了します。
This completes the creation of the database for Blast search.
Blast検索結果ダイアログが表示されます。
The Blast search results dialog is displayed.
実行確認ダイアログが拡大し表示され、Blast検索実行パラメータが表示されます。
The execution confirmation dialog is expanded and displayed, and Blast search execution parameters are displayed.
The nucleotide sequence and amino acid sequence of the genomic sequence loaded in the current referencedirectory can be selected as a database for Blast search.
This shows that two types of Blast search databases are generated from the loaded genomic sequence and are automatically registered as a base sequence database and as an amino acid sequence database.
Please check this out for details BL color button: When a Blast search result is given to each feature to be drawn, it is drawn with the fill color assigned to that score range.
The timing for generating the Blast search database is when the new base sequence file is loaded into the current reference directory and when the current reference directory is changed.
In IMCGE, AE, and DS, multiple genome sequences can be loaded simultaneously into the current reference directory,so multiple Blast search databases are generated at the same time.
Click"Show Parameter" on this dialog to display the Blast search parameters and change the Blast search execution parameters. Blast search execution parameters will be explained separately.
IMC E04A1 Blast Search for Reference Genome by Entering Query Sequence Specify it by directly entering or pasting the query sequence and performing homology search on the sequences loaded in the current main directroy, current reference directory and on registered databases.
About VecScreen VecScreen is a tool that can detect foreign DNAs such as vector, linker, adapter, and primer regions(we call vector contamination in this document) involved in nucleotide sequences(query)by using blast search against vector sequence database.
これでローカルBlast検索用のデータベースが生成されました。
A database for local blast search has been generated.
マウスをドラッグして、Blast検索を実行する領域を選択します。
Drag the mouse and select the area to perform Blast search.
Perform a Blast search on the genomic base sequence and amino acid sequence on the current reference directory, with one feature on the main feature map as the query sequence.
例として、COGアミノ酸データファイルをBlast検索できるようにBlastデータベース化します。
As an example, we will create a Blast database so that the COG amino acid data file can be searched for in Blast..
Sequencer table- RPD| LSDB Archive Using the amino acid sequencedetermined by gas-phase protein sequencer as query, BLAST search was performed to NR database restricted to Oryza sativa.
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