Examples of using Disc diffusion in English and their translations into Portuguese
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Antimicrobial susceptibility profile was determined by disc diffusion method and etest.
Disc diffusion, MIC determination and latex agglutination are all sufficient and affordable methods for routine methicillin sensitivity testing.
The in vitro antibacterial assays were conducted by the disc diffusion method.
The antifungigram was performed using the disc diffusion technique with the antifungal agents nystatin, amphotericin b, miconazole and fluconazole, evaluating them in isolation and associated with oe. mic and mfc rang.
The assessment of their sensitivity profile was performed using the standard disc diffusion method.
Using the disc diffusion method was observed a resistance rate above 50% to antimicrobials often used in clinical routine like erythromycin, ciprofloxacin and clindamycin.
All positive strains were subjected to antimicrobial susceptibility testing by the disc diffusion method.
The disc diffusion method, using oxacillin or cefoxitin discs, is popular in some countries, whereas Etest strips are generally considered too expensive for routine use.
The analysis of susceptibility to 15 antimicrobials was performed by the disc diffusion method with the muller hinton agar.
Penicillin susceptibility was assessed by means of the oxacillin disc diffusion method 1 µg, with an inhibition zone> 20 mm considered indicative of susceptibility to penicillin, and a halo< 19 mm, as resistance to penicillin.
All the isolates of S. equi demonstrated sensitivity to penicillin, both by the disc diffusion method and microdilution method.
The antibacterial activity was also demonstrated by disc diffusion method, getting inhibition of bacterial growth between 9-23 mm in diameterin the following concentrations 512, 256, 128 e 64¿g/ml.
Aeruginosa from the hospital wastewater treatment plant- hwtp(n 29 of influent andn 28 of effluent) were analyzed for antimicrobial susceptibility by disc diffusion method.
VISA and VRSA, for example,are not reliably detected using automated methods, whereas disc diffusion is inappropriate for VISA, but can be used for VRSA.
The disc diffusion method for the assessment of antimicrobial susceptibility in 18 strains of coagulasis-positive staphylococcus, 22 strains of staphylococcus and escherichia coli isolated from milk samples.
Still as control test,bacteria with phenotypes resistant to oxacillin isolated by means of the disc diffusion technique were submitted to a ciprofloxacin sensitivity test.
A total of 225 salmonella isolates from feed mills, lairage environment, carcasses andintestinal content of the pigs were tested for antimicrobial susceptibility against twelve different antimicrobial agents by agar disc diffusion.
The bacterial species were identified by biochemical tests; andthe antimicrobial susceptibility through the disc diffusion technique or by the identification of the Minimum Inhibitory Concentration MIC.
The isolation and identification of bacteria were performed by an outsourced laboratory,through the method standardized by the clinical and laboratory standards institute(clsi) and for the evaluation of the antimicrobial susceptibility was used the disc diffusion method.
The samples were subjected to antimicrobial susceptibility profile for disc diffusion method, detection of colotimétrica hydrolysis of imipenem(carba np), polymerase chain reaction(pcr) for the detection of carbapenemases genes(imp, vim, ndm, spm, kpc, ges, oxa-48), genes of resistance to aminoglycosides.
Clinical and Laboratory Standards Institute™(CLSI),formerly NCCLS breakpoints are presented in the below table for MIC testing(mg/ l) or disc diffusion testing(zone diameter[mm]) using a 5-µg moxifloxacin disc. .
There are many options for testing methicillin susceptibility of S. aureus, including disc diffusion, MIC measurements in broth or by Etest, chromogenic agar, latex agglutination, automated methods, rapid screening methods and molecular approaches see Table 4 for details.
Initially it was determined the antimicrobial activity of seven genotypes of essential oil by disc diffusion technique on the bacteria pseudomonas aeruginosa, staphylococcus aureus, bacillus cereus, bacillus subtilis, serratia marcescens, escherichia coli, enterococcus faecalis and salmonella enteritidis.
The BSAC recommends Columbia or Mueller Hinton agar supplemented with NaCl 2% for dilution and disc diffusion methods, and addition of up to 5% NaCl to media has been shown to improve detection of resistance for most strains. Typically, methicillin resistance is detected more reliably at lower temperatures 30-35ºC, although some rare strains may grow slowly at 30ºC when 5% NaCl is present.
The extracts obtained were submitted to two methodologies used to determine antifungal activity: agar diffusion(disc) and agar dilution, against biological samples of: candida albicans atcc 18804; candida tropicalis atcc 750; candida parapsilosis atcc 22019, candida glabrata atcc 2001and candida krusei atcc 2742, belonging to pathogenic fungi collection of the mycology laboratory of the federal university of minas gerais. besides these agents.
The diffusion in disc test was made with etanolic extract and eluate.
Dilution broth tests were made only with extracts that revealed the bests results in diffusion disc test.
The antibacterial activity were investigated against the gram positive microorganisms S. pneumoniae and S. aureus and the gram negative microganisms E. coli andP. aeruginosa in diffusion in disc test and dilution broth test.