Polyplus-transfection applies its 15+ year expertise to the development of novel transfection solutions for mammalian cells.
转染的”或“转化的”或“转导的”细胞是已经用外源核酸转染,转化或转导的细胞。
A"transfected" or"transformed" or"transduced"cell is one which has been transfected, transformed or transduced with exogenous nucleic acid.
这一集成的系统实现了整个转染过程,并通过光学显微镜对转染后的细胞反应进行监测。
This integrated system allows the entire transfection process and post-transfection cell response to be monitored by an optical microscope.
此外,研究表明转染质粒被整合到寄主基因组,因此它们仍构成插入突变的潜在风险。
Furthermore, transfected plasmids have been shown to integrate into the host genome and therefore they still pose the risk of insertional mutagenesis.
此外,转染的细胞的百分比将超额表达荧光蛋白,经常点创建一个潜在的毒性作用。
In addition, a percentage of the transfected cells will over-express the fluorescent protein, often to the point of creating a potentially toxic effect.
这两种传感器都可以通过转染或转导的方式表达成各种细胞类型,并且可以针对特定的细胞室。
Both sensors canbe expressed into various cell types via transfection or transduction, and can be targeted to specific cellular compartments.
成功的哺乳动物转染实验依赖于使用的高品质的质粒或病毒DNA载体,是相对自由的细菌内毒素。
Successful mammalian transfection experiments rely on the use of high quality plasmid or viral DNA vectors that are relatively free of bacterial endotoxins.
该系统技术简单,利用质粒转染或电转的方法可将您感兴趣的基因永久整合到宿主基因组中。
This system is technically simple, utilizing plasmid transfection or electroporation to permanently integrate your gene(s) of interest into the host genome.
转染效率可通过测定细胞的密度,转染的时间和siRNA与转染试剂的比例来评价。
Transfection efficiency is optimized by titrating cell density, transfection time, and the ratio of siRNA to transfection reagent.
非分裂细胞比分裂细胞更难转染,原代细胞比永生化细胞系更难转染。
Non-dividing cells are often more difficult to transfect than dividing cells, and primary cells are often harder to transfect than immortalized cell lines.
除成熟红血球之外,几乎每个细胞输入您的身体,认可SNAs和迅速地向内他们,不用对转染作用者的需要。
Almost every cell type in your body, other than mature red blood cells,recognize SNAs and rapidly internalize them without the need for transfection agents.
However, conventional HBV vaccination strategies rely on intramuscular administration of the small hepatitis B surface(SHBs)protein produced from transfection in S. cerevisiae cells.
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