Examples of using Stringency in English and their translations into Japanese
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The difference between the two flags is stringency.
A final low stringency wash can be conducted in 0.1×SSC at room temperature.
Post-hybridization washes generally determine stringency conditions.
Wash stringency conditions can be defined by salt concentration and by temperature.
In general,salt and/or temperature can be altered to change stringency.
I smiled and said,“It was good, except that stringency was more felt than affection.”.
In general,salt and/or temperature can be altered to change stringency.
As above, wash stringency can be increased by decreasing salt concentration or by increasing temperature.
Disinfection, diminish inflammation, hemostasia, sterilization and con- stringency.
Low stringency conditions" are, for example, 5×SSC, 5×Denhardt's solution, 0.5% SDS, 500% formamide at 32° C.
All of the foregoing hybridizations wouldbe considered to be under conditions of high stringency.
A specific example of“low stringency” hybridization conditions is when the above hybridization is conducted at 10% formamide.
As known in the art, numerous equivalent conditions may be employed to comprise either low orhigh stringency conditions.
Low stringency conditions can utilize a hybridization and/or wash at 11, 12, 13, 14, 15, or 20°C lower than the Tm.
For certain applications, for example, site-directed mutagenesis,it is appreciated that lower stringency conditions are preferred.
By controlling the stringency of the hybridization and/or washing conditions, target sequences that are 100% complementary to the probe can be identified.
The concentration and types of these additivescan be changed without substantially affecting the stringency of the hybridization conditions.
The stringency and applicability dates, which the CO2 standard imposes, will depend on the weight of the aircraft and whether it concerns a“new type” aircraft or an“in-production” aircraft.
The concentration and types of these additivescan be changed without substantially affecting the stringency of the hybridization conditions.
As used herein, the term"hybridizes under conditions of low stringency, medium stringency, high stringency or very high stringency" describes hybridization and washing conditions.
For applications requiring high selectivity,one will typically desire to employ relatively high stringency conditions to form the hybrids.
As used herein, the term"hybridizes under low stringency, medium stringency, high stringency, or very high stringency conditions" describes conditions for hybridization and washing.
Electronic stringency hybridization of the target is one distinctive aspect of this method since it is amenable with double stranded DNA and results in rapid and precise hybridization of the target to the capture.
The term"stringent conditions" as used herein may be any of low stringency conditions, moderate stringency conditions or high stringency conditions.
The hybridization reaction may also include another agent affecting the stringency, for example, hybridization conducted in the presence of 50% formamide increases the stringency of hybridization at a defined temperature.
In addition, the temperature in the wash step can be increased from low stringency conditions at room temperature, aboiit 22° C to high stringency conditions at about 65° C. Both temperature.
Washing can be carried out using only one of these conditions, for example,high stringency conditions, or each of the conditions can be used, for example, for 10 to 15 minutes each, in the order listed above, repeating any or all of the steps listed.